Staining

• Neurofillament immunostaining (wholemount)
• X-gal staining
• BrdU Immunohistochemistry
• BrdU immunofluorescence
• TUNEL assay
• Immunofluorescence
• Wholemount in situ Hybridization
• Section in situ Hybridization
• Synthesis of Dig-labeled RNA Probe
• Immunohistochemistry
• H&E Staining
• Nissl Staining

BAC modification

• Preparation of electrocompetent cells
• BAC DNA isolation
• Mini prep of BAC DNA
• Large prep of BAC DNA
• Preparationof recombination competent EL350/EL250 cells
• Retrieving DNA by Gap Repairing
• Prep BAC DNA for pronuclear injection

Mouse Embryonic Stem (ES) Cell Manipulations

• ES cell culture
• Mini-Southern blot analysis
• Preparation of feeder cells
• STO cell feeder plate
• Gene targeting in ES cells

Manipulations of Mouse Embryos

• Morula aggregation
• Superovulation
• Fertilized eggs collection
• Preparation of DNA fragment for production of trasngenic mouse
• PCR genotyping

Manipulation of Chick embryos

• Electroporation in chick embryos

Molecular Biology

• Mini-prep of plasmid DNA
• Colony screening by PCR
• Preparation of chemical competent cells
• Preparation of electro-competent cells
• DNA probe labeling for Southern
• Heat shock transformation
• "One minute" transformation for purified plasmid DNA
• Transformation by electroporation
• Total RNA extraction